CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0002 details


CrisprIDCrispr0002
OrganismHomo sapiens
Gene/Locus (Literature)CLTA1
NCBI Gene NameCLTA
Target SequenceGCAGATGTAGTGTTTCCACAGGG
Target+Mutant_Flanking

ATTGCAGATGTAGTGTTTCCACAGGGTGGCTCTTCAGTGCACCAGCGGGGCC
ATTGCAGATGTAGTGTTTCC
- - - -- - - GGCTCTTCAGTGCACCAGCGGGGCC

Modification LengthD7
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 2/0.5
Cell line HEK293T Cells
Method/AssaySurveyor assay
Crispr efficiency 6-8%
PRM/crRNA/VectorU6
PRM-cas/vectorCMV/Cas-HA-NLS-GFP
Pubmed23386978