CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0003 details


CrisprIDCrispr0003
OrganismHomo sapiens
Gene/Locus (Literature)CLTA1
NCBI Gene NameCLTA
Target SequenceGCAGATGTAGTGTTTCCACAGGG
Target+Mutant_Flanking

ATTGCAGATGTAGTGTTTCCACAGGGTGGCTCTTCAGTGCACCAGCGGGGCC
ATTGCAGATGTAGTGTTTCC ACAGGGT - - - - - - - - - - - - -- - - - - - - - - - GCC

Modification LengthD22
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 2/0.5
Cell line HEK293T Cells
Method/AssaySurveyor assay
Crispr efficiency 6-8%
PRM/crRNA/VectorU6
PRM-cas/vectorCMV/Cas-HA-NLS-GFP
Pubmed23386978