CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0005 details


CrisprIDCrispr0005
OrganismRattus norvegicus
Gene/Locus (Literature)ApoE
NCBI Gene NameApoE
Target SequenceCCTGAACCGCTTCTGGGATTACC
Target+Mutant_Flanking

CTGGGAGCAGGCCCTGAACCGCTTCTGGGATTACCTGCGCTGGGTGC
CTGGGA - - - - - - - - - - - - -- - - - - - - - - - -TTACCTGCGCTGGGTGC

Modification LengthD24
Genetic ModificationDeletion
LocationExon3
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency 73.30%
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943