CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0006 details


CrisprIDCrispr0006
OrganismRattus norvegicus
Gene/Locus (Literature)B2m
NCBI Gene NameB2m
Target SequenceCCTTCAGCAAGGACTGGTCTTTC
Target+Mutant_Flanking

TGTCAGATCTGTCCTTCAGCAAGGACTGGTCTTTCTACATCCTGGCT
TGTCAGATCTGTCCTTC - - - t - - - ACTGGTCTTTCTACATCCTGGCT

Modification LengthD7,+1
Genetic ModificationIndels
LocationExon2
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency 60%
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943