A Central Hub of Crispr based Genome Editing
CrisprID | Crispr0007 |
Organism | Rattus norvegicus |
Gene/Locus (Literature) | B2m |
NCBI Gene Name | B2m |
Target Sequence | CCTTCAGCAAGGACTGGTCTTTC |
Target+Mutant_Flanking | TGTCAGATCTGTCCTTCAGCAAGGACTGGTCTTTCTACATCCTGGCT |
Modification Length | D3 |
Genetic Modification | Deletion |
Location | Exon2 |
Conc. (ng/μl) | 10/20ng |
Cell line | Zygote |
Method/Assay | T7E1 assay |
Crispr efficiency | 60% |
PRM/crRNA/Vector | pUC57-sgRNA |
PRM-cas/vector | T7 |
Pubmed | 24598943 |