CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0008 details


CrisprIDCrispr0008
OrganismRattus norvegicus
Gene/Locus (Literature)Prf1
NCBI Gene NamePrf1
Target SequenceCTGCTAATATCAATAACGACTGG
Target+Mutant_Flanking

CGCGGGAGGCGGCTGCTAATATCAATAACGACTGGCGTGCGGGGCTG
CGCGGGAGGCGGCTGCTAATATCAATAAC - c - - GGCGTGCGGGGCTG

Modification LengthD4,+1
Genetic ModificationIndels
LocationExon2
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency 26.70%
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943