CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0009 details


CrisprIDCrispr0009
OrganismRattus norvegicus
Gene/Locus (Literature)Prkdc
NCBI Gene NamePrkdc
Target SequenceCGAGCTGTTCAGAAACACCAAGG
Target+Mutant_Flanking

AGCTTCAGGGATCGAGCTGTTCAGAAACACCAAGGACTGAAGCTTGC
AGCTTCAGGGATCGAGCTGTTCAGAA - - -- - - - - - - - - - - - GCTTGC

Modification LengthD15
Genetic ModificationDeletion
LocationExon23
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency 66.70%
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943