CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0032 details


CrisprIDCrispr0032
OrganismRattus norvegicus
Gene/Locus (Literature)ApoE
NCBI Gene NameApoE
Target SequenceCCTGAACCGCTTCTGGGATTACCTG . . . 27bp . . . GTCCAGGAAGAGCTGCAGAGCTCCC
Target+Mutant_Flanking

GGGAGCAGGCCCTGAACCGCTTCTGGGATTACCTG . . . 27bp . . . GTCCAGGAAGAGCTGCAGAGCTCCCAAG
GGGAGCAGGCCCTGAACcCGCTTCTGGGATTACCTG . . .27bp . . . GTCCAGGAaaAGAGCTGCAGAGCTCCCAAG

Modification Lengthins1,+2
Genetic ModificationInsertion
LocationExon3
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943