CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0064 details


CrisprIDCrispr0064
OrganismRattus norvegicus
Gene/Locus (Literature)B2m
NCBI Gene NameB2m
Target SequenceCCAAATTCAAGTGTACTCTCGCCATCCA . . . 116bp . . . TCTGTCCTTCAGCAAGGACTGGTCTTTC
Target+Mutant_Flanking

TCCCCAAATTCAAGTGTACTCTCGCCATCCA . . . 116bp . . .TCTGTCCTTCAGCAAGGACTGGTCTTTCTAC
TCCCCAAATTCAAGTGTACTCTCGCCATCCA .. . 116bp . . . TCTGTCCTTc - - - CAAGGACTGGTCTTTCTAC

Modification LengthD4,+1
Genetic ModificationIndels
LocationExon2
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943