CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0110 details


CrisprIDCrispr0110
OrganismRattus norvegicus
Gene/Locus (Literature)Prf1
NCBI Gene NamePrf1
Target SequenceCTCACACTGCCAGCGTAATGTGGCCACAA . . . 30bp . . . AGGCGGCTGCTAAATATCAATAACGACTGG
Target+Mutant_Flanking

CGGCTCACACTGCCAGCGTAATGTGGCCACAA . . . 30bp . . .AGGCGGCTGCTAAATATCAATAACGACTGGCGT
CGGCTCACACTGCCAGCGTAATGTGGCCACAA . . . 30bp . . .AGGCGGCTGCTAAATATCA - - - -- gtCTGGCGT

Modification LengthD7,+2
Genetic ModificationIndels
LocationExon2
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943