CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0113 details


CrisprIDCrispr0113
OrganismRattus norvegicus
Gene/Locus (Literature)Prf1
NCBI Gene NamePrf1
Target SequenceCTCACACTGCCAGCGTAATGTGGCCACAA . . . 30bp . . . AGGCGGCTGCTAAATATCAATAACGACTGG
Target+Mutant_Flanking

CGGCTCACACTGCCAGCGTAATGTGGCCACAA . . . 30bp . . .AGGCGGCTGCTAAATATCAATAACGACTGGCGT
CGGCTCACACTGCCAGC - - -- - - - - - - - - - - - - . . . 30bp . . .  - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - -

Modification LengthD154
Genetic ModificationDeletion
LocationExon2
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943