A Central Hub of Crispr based Genome Editing
CrisprID | Crispr0140 |
Organism | Rattus norvegicus |
Gene/Locus (Literature) | Prkdc |
NCBI Gene Name | Prkdc |
Target Sequence | CGAGCTGTTCAGAAACACCAAGGACTGA . . . 24bp . . . ACTGGAGGAAGTGTGATTCATGGTGG |
Target+Mutant_Flanking | GATCGAGCTGTTCAGAAACACCAAGGACTGA . . . 24bp . . . ACTGGAGGAAGTGTGATTCATGGTGGGCC |
Modification Length | D13,+32 |
Genetic Modification | Indels |
Location | Exon23 |
Conc. (ng/μl) | 10/20ng |
Cell line | Zygote |
Method/Assay | T7E1 assay |
Crispr efficiency | NA |
PRM/crRNA/Vector | pUC57-sgRNA |
PRM-cas/vector | T7 |
Pubmed | 24598943 |