CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0146 details


CrisprIDCrispr0146
OrganismRattus norvegicus
Gene/Locus (Literature)Prkdc
NCBI Gene NamePrkdc
Target SequenceCGAGCTGTTCAGAAACACCAAGGACTGA . . . 24bp . . . ACTGGAGGAAGTGTGATTCATGGTGG
Target+Mutant_Flanking

GATCGAGCTGTTCAGAAACACCAAGGACTGA . . . 24bp . . . ACTGGAGGAAGTGTGATTCATGGTGGGCC
GATCGAGCTGTTCAGA - - - - - - - - - - ACTGA. . . 24bp . . . ACTGGAGGAAGT - - - - - - -- - GGTGGGCC

Modification LengthD10,D9
Genetic ModificationDeletion
LocationExon23
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943