CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0161 details


CrisprIDCrispr0161
OrganismRattus norvegicus
Gene/Locus (Literature)Prkdc
NCBI Gene NamePrkdc
Target SequenceCGAGCTGTTCAGAAACACCAAGGACTGA . . . 24bp . . . ACTGGAGGAAGTGTGATTCATGGTGG
Target+Mutant_Flanking

GATCGAGCTGTTCAGAAACACCAAGGACTGA . . . 24bp . . . ACTGGAGGAAGTGTGATTCATGGTGGGCC
GATCGAGCTGTTCAGAAACACttcctccagttttgcagaattgcagtcgcaagcttcagtcct - - - - - - - - - - - - -  . . . 24bp . . . - - - - - - - - - - - - -- - - - - - - - - - -TGGTGGGCC

Modification LengthD54,+42
Genetic ModificationIndels
LocationExon23
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943