CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0185 details


CrisprIDCrispr0185
OrganismRattus norvegicus
Gene/Locus (Literature)Prkdc
NCBI Gene NamePrkdc
Target SequenceCGAGCTGTTCAGAAACACCAAGGACTGA . . . . . . ACTGGAGGAAGTGTGATTCATGGTGG
Target+Mutant_Flanking

GATCGAGCTGTTCAGAAACACCAAGGACTGA . . . . . . ACTGGAGGAAGTGTGATTCATGGTGGGCC
GATCGAGCTGTTCAaAAACACCAAGGACTGA . . . . . .ACTGGAGGAA - - - - - - - - - - - - GTGGGCC

Modification LengthD12,m1
Genetic ModificationDeletion/point mutation
LocationExon23
Conc. (ng/μl) 10/20ng
Cell line Zygote
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpUC57-sgRNA
PRM-cas/vectorT7
Pubmed24598943