CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0220 details


CrisprIDCrispr0220
OrganismHomo sapiens
Gene/Locus (Literature)HBB
NCBI Gene NameHBB
Target SequenceCACGTTCACCTTGCCCCACAGGG
Target+Mutant_Flanking

ACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCTCCTCAGGAG
ACCACCAACTTCA - - - -- - - - - - - - - - - - - - - - - - GGGCAGTAACGGCAGACTTCTCCTCAGGAG

Modification LengthD22
Genetic ModificationDeletion
LocationExon
Conc. (ng/μl) 100/100ng
Cell line HEK293T Cells
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 70%
PRM/crRNA/VectorpX330/U6
PRM-cas/vectorpX330
Pubmed23939622