A Central Hub of Crispr based Genome Editing
CrisprID | Crispr0228 |
Organism | Homo sapiens |
Gene/Locus (Literature) | HBB |
NCBI Gene Name | HBB |
Target Sequence | CACGTTCACCTTGCCCCACAGGG |
Target+Mutant_Flanking | ACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCTCCTCAGGAG |
Modification Length | 9 |
Genetic Modification | Insertion |
Location | Exon |
Conc. (ng/μl) | 100/100ng |
Cell line | HEK293T Cells |
Method/Assay | T7E1 assay/Sequencing |
Crispr efficiency | 70% |
PRM/crRNA/Vector | pX330/U6 |
PRM-cas/vector | pX330 |
Pubmed | 23939622 |