CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0238 details


CrisprIDCrispr0238
OrganismHomo sapiens
Gene/Locus (Literature)HBB
NCBI Gene NameHBB
Target SequenceCTTGCCCCACAGGGCAGTAACGG
Target+Mutant_Flanking

TTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCTCCTCAGGAGTCAGGTGCA
TTCATCCACGTTCACCTTGCCCC - - - - - - - - - - TAACGGCAGACTTCTCCTCAGGAGTCAGGTGCA

Modification LengthD10
Genetic ModificationDeletion
LocationExon
Conc. (ng/μl) 100/100ng
Cell line HEK293T Cells
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 77%
PRM/crRNA/VectorpX330/U6
PRM-cas/vectorpX330
Pubmed23939622