CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0289 details


CrisprIDCrispr0289
OrganismHomo sapiens
Gene/Locus (Literature)CCR5
NCBI Gene NameCCR5
Target SequenceGCTGCCGCCCAGTGGGACTTTGG
Target+Mutant_Flanking

GTCCCCTTCTGGGCTCACTATGCTGCCGCCCAGTGGGACTTTGGAAATACAATGTGTCAACTCTT
GTCCCCTTCT - GGCTCACTATGCTGCCGCCCAGTGGGACTTTGGAAATACAATGTGTCAACTCTT

Modification LengthD1
Genetic ModificationDeletion
LocationExon
Conc. (ng/μl) 100/100ng
Cell line HEK293T Cells
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 76%
PRM/crRNA/VectorpX330/U6
PRM-cas/vectorpX330
Pubmed23939622