CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0327 details


CrisprIDCrispr0327
OrganismNicotiana benthamiana
Gene/Locus (Literature)PDS
NCBI Gene NamePDS
Target SequenceGCTTTTCCCTGATGAAATTTCGG
Target+Mutant_Flanking

AATTATTGATGCTACAATGAAGGAACTAGCAAAGCTTTTCCCTGATGAAATTTCGGCAGATCAGAGCAAAGCAAA
AATTATTGATGCTACAATGAAGGAACTAGCAAAGCTTTTCCCTGATGA
- - - TTCGGCAGATCAGAGCAAAGCAAA

Modification LengthNA
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 1:1 ratio
Cell line Leaf
Method/AssayPCR/Sequencing
Crispr efficiency 12.70%
PRM/crRNA/VectorCaMV 35S
PRM-cas/vectorCaMV 35S
Pubmed24122057