CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0354 details


CrisprIDCrispr0354
OrganismDanio rerio
Gene/Locus (Literature)nsd2
NCBI Gene NameWHSC1
Target SequenceGGAAGCAAGTACCAGCAGACTGG
Target+Mutant_Flanking

AGACAGGGGAAGCAAGTACCAGCAGACTGGCATTGGGAAGGTCTTTAA
AG - - - - - - - - - - - - - - - - CATTGGGACTGGCATTGGGAAGGTCTTTAA

Modification LengthD22,+6
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) 5:3 ratio
Cell line Embryo
Method/AssayqPCR
Crispr efficiency NA
PRM/crRNA/VectorPcs2-cas9
PRM-cas/vectorPT7
Pubmed24901507