A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr0366 |
| Organism | Solanum lycopersicum |
| Gene/Locus (Literature) | H9 DNMT3b-tdTomato |
| NCBI Gene Name | DNMT3B |
| Target Sequence | GTACGTGAAGCACCCCGCCGACATCCCCGATT |
| Target+Mutant_Flanking | CCAAGGCGTACGTGAAGCACCCCGCCGA - - -- - - - - - - CATCCCCGATTACAA |
| Modification Length | 1 |
| Genetic Modification | Insertion |
| Location | NA |
| Conc. (ng/μl) | 7.5/7.5 |
| Cell line | HEK293T Cells |
| Method/Assay | Sequencing |
| Crispr efficiency | 5.40% |
| PRM/crRNA/Vector | U6 |
| PRM-cas/vector | P-simpleII-NLS-Nmcas9-HA-NLS |
| Pubmed | 23940360 |