CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0372 details


CrisprIDCrispr0372
OrganismSolanum lycopersicum
Gene/Locus (Literature)H9 DNMT3b-tdTomato
NCBI Gene NameDNMT3B
Target SequenceGTACGTGAAGCACCCCGCCGACATCCCCGATT
Target+Mutant_Flanking

CCAAGGCGTACGTGAAGTCACCCCGCCGA - -- - - - - - - - CATCCCCGATTACAA
CCAAGGCGTACGTGAAGTAC
GTGAAG - - - - - - - - - - - - CATCCCCGATTACAA

Modification LengthD12,+6
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) 7.5/7.5
Cell line HEK293T Cells
Method/AssaySequencing
Crispr efficiency 5.40%
PRM/crRNA/VectorU6
PRM-cas/vectorP-simpleII-NLS-Nmcas9-HA-NLS
Pubmed23940360