A Central Hub of Crispr based Genome Editing
CrisprID | Crispr0372 |
Organism | Solanum lycopersicum |
Gene/Locus (Literature) | H9 DNMT3b-tdTomato |
NCBI Gene Name | DNMT3B |
Target Sequence | GTACGTGAAGCACCCCGCCGACATCCCCGATT |
Target+Mutant_Flanking | CCAAGGCGTACGTGAAGTCACCCCGCCGA - -- - - - - - - - CATCCCCGATTACAA |
Modification Length | D12,+6 |
Genetic Modification | Indels |
Location | NA |
Conc. (ng/μl) | 7.5/7.5 |
Cell line | HEK293T Cells |
Method/Assay | Sequencing |
Crispr efficiency | 5.40% |
PRM/crRNA/Vector | U6 |
PRM-cas/vector | P-simpleII-NLS-Nmcas9-HA-NLS |
Pubmed | 23940360 |