CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0389 details


CrisprIDCrispr0389
OrganismMus musculus
Gene/Locus (Literature)Tet2
NCBI Gene NameTet2
Target SequenceGGAGCTGAGCCAAAAGAGGTTGG
Target+Mutant_Flanking

AGGAAATTTAGGCTACATGGAGCTGAGCCAAAAGAGGTTGGTTTGTTTCCTAATCTATAA
AGGAAATTTAGGCTACATGGAGCTGAGCCA
ttggtatt - - -- - - - GGTTGGTTTGTTTCCTAATCTATAA

Modification LengthD7,+8
Genetic ModificationIndels
LocationExon9
Conc. (ng/μl) NA
Cell line hESCs
Method/AssayPCR/Sequencing
Crispr efficiency 30%
PRM/crRNA/VectorU6
PRM-cas/vectorCAG
Pubmed24432195