CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0488 details


CrisprIDCrispr0488
OrganismHomo sapiens
Gene/Locus (Literature)CCR5
NCBI Gene NameCCR5
Target SequenceTGACATCAATTATTATACATCGGAGCCCTG . . . / / . . . GGGGGCCAGACTCCCCATGTCTCTAGGT
Target+Mutant_Flanking

GTCCAATCTATGACATCAATTATTATACATCGGAGCCCTG . . . / / . . .GGGGGCCAGACTCCCCATGTCTCTAGGTCAATACATGTGTGTCC
GTCCAATCTATGACATCAATTATTA - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - TAGGTCAATACATGTGTGTCC

Modification LengthNA
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 20/15
Cell line K562 cells
Method/AssayT7E1 assay
Crispr efficiency 12%
PRM/crRNA/VectorT7
PRM-cas/vectorPET28-b(+)
Pubmed24696461