CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0510 details


CrisprIDCrispr0510
OrganismHomo sapiens
Gene/Locus (Literature)AAVS1-AS2
NCBI Gene NameAAVS1
Target SequenceCTCCCTCCCAGGATCCTCTCTGG
Target+Mutant_Flanking

AAGCTCTCCCTCCCAGGATCCTCTCTGGCTCCATCGTAAGCA
AAGCTCTCCCTCCCAGGA
- - - -- TCTGGCTCCATCGTAAGCA

Modification LengthD5
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 20/15
Cell line K562 cells
Method/AssayT7E1 assay
Crispr efficiency 72%
PRM/crRNA/VectorT7
PRM-cas/vectorPET28-b(+)
Pubmed24696461