CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0526 details


CrisprIDCrispr0526
OrganismHomo sapiens
Gene/Locus (Literature)OPN1SW
NCBI Gene NameOPN1SW
Target SequenceATTCTGGTCAACGTGTCCTTCGG
Target+Mutant_Flanking

ACTACATTCTGGTCAACGTGTCCTTCGGAGGCTTCCTCCTCT
ACTACATTCTGG
- caac - - - - - CTTCGGAGGCTTCCTCCTCT

Modification LengthD10,+4
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) 20/15
Cell line K562 cells
Method/AssayT7E1 assay
Crispr efficiency 56%
PRM/crRNA/VectorT7
PRM-cas/vectorPET28-b(+)
Pubmed24696461