CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0527 details


CrisprIDCrispr0527
OrganismHomo sapiens
Gene/Locus (Literature)OPN1SW
NCBI Gene NameOPN1SW
Target SequenceATTCTGGTCAACGTGTCCTTCGG
Target+Mutant_Flanking

ACTACATTCTGGTCAACGTGTCCTTCGGAGGCTTCCTCCTCT
ACTACATTCTGGTCAACGT
- g - - TCGGAGGCTTCCTCCTCT

Modification LengthD4,+1
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) 20/15
Cell line K562 cells
Method/AssayT7E1 assay
Crispr efficiency 56%
PRM/crRNA/VectorT7
PRM-cas/vectorPET28-b(+)
Pubmed24696461