A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr0539 |
| Organism | Oreochromis niloticus |
| Gene/Locus (Literature) | nanos2 |
| NCBI Gene Name | nanos2 |
| Target Sequence | GGACTGCTTTAACATGTGGCAGG |
| Target+Mutant_Flanking | GGACTGCTTTAACATGTGGCAGGACTACATGA |
| Modification Length | 5 |
| Genetic Modification | Insertion |
| Location | Exon1 |
| Conc. (ng/μl) | 50/500 |
| Cell line | Embryo |
| Method/Assay | Sequencing |
| Crispr efficiency | 38% |
| PRM/crRNA/Vector | PMD-19T/T7 |
| PRM-cas/vector | PcDNA3.1 |
| Pubmed | 24709635 |