CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0615 details


CrisprIDCrispr0615
OrganismDaphnia
Gene/Locus (Literature)dma-ey
NCBI Gene Nameey
Target SequenceCCCACGCCGACGACAACAACACCGGCGTCGTGAGGAGAAATTACGG
Target+Mutant_Flanking

GGCGAGAATTCTCGGTCGGCTCCGCCCACGCCGACGACAACAACACCTTCG (365) ACTAAT (45) AATGGCGTCGTGAGGAGAAATTACGGAATCA (401) CAGC
GGCGAGAATTCTCGGTCGGCTCCGCCCACGCCGACGACAACAACACCTTCG(365) ACTAAT (45) AATGGCGTC - - - -- - - - - -AATTACGGAATCA (401) CAGC

Modification LengthD10
Genetic ModificationDeletion
LocationExon8- Exon10
Conc. (ng/μl) 50/500
Cell line Daphnia eggs
Method/AssayPCR
Crispr efficiency 18-47%
PRM/crRNA/VectorpDR274
PRM-cas/vectorPCS-Dmavas-cas9
Pubmed24878568