CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0617 details


CrisprIDCrispr0617
OrganismDaphnia
Gene/Locus (Literature)dma-ey
NCBI Gene Nameey
Target SequenceCCCACGCCGACGACAACAACACCGGCGTCGTGAGGAGAAATTACGG
Target+Mutant_Flanking

GGCGAGAATTCTCGGTCGGCTCCGCCCACGCCGACGACAACAACACCTTCG (365) ACTAAT (45) AATGGCGTCGTGAGGAGAAATTACGGAATCA (401) CAGC
GGCGAGAATTTCGCTCGGTCGGCTCCCACGAGAATTC - - - - - - - - - - - TCG (365) ACTAAT (45)AATGGCGTCGTGAGGAGAAATTACGGAATCA (401) CAGC

Modification LengthD18,+7
Genetic ModificationIndels
LocationExon8- Exon10
Conc. (ng/μl) 50/500
Cell line Daphnia eggs
Method/AssayPCR
Crispr efficiency 18-47%
PRM/crRNA/VectorpDR274
PRM-cas/vectorPCS-Dmavas-cas9
Pubmed24878568