CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0728 details


CrisprIDCrispr0728
OrganismSurrogate reporter
Gene/Locus (Literature)F13A1
NCBI Gene NameF13A1
Target SequenceACAAGCTGATTGTCCGCAGAGGG
Target+Mutant_Flanking

CAAGTATGAAAACAACAAGCTGATTGTCCGC - AGAGGGCAGTCTTTCTAT
CAAGTATGAAAACAACAAGC
- - a - - - - - - - - - AGAGGGCAGTCTTTCTAT

Modification LengthD11,+1
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) NA
Cell line HEK293T Cells
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 32%
PRM/crRNA/VectorPRG2-CT/hU6
PRM-cas/vectorP3S-CasHC
Pubmed24569644