A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr0731 |
| Organism | Surrogate reporter |
| Gene/Locus (Literature) | PPP1R1B |
| NCBI Gene Name | PPP1R1B |
| Target Sequence | CCATCTCAAGTCGAAGAGACCCA |
| Target+Mutant_Flanking | CCTCAGGAGAGGGGCACCATCT - - - - - - CAAGTCGAAGAGACCCAACCCCTGTG |
| Modification Length | 40 |
| Genetic Modification | Insertion |
| Location | NA |
| Conc. (ng/μl) | NA |
| Cell line | HEK293T Cells |
| Method/Assay | T7E1 assay/Sequencing |
| Crispr efficiency | 23% |
| PRM/crRNA/Vector | PRG2-CT/hU6 |
| PRM-cas/vector | P3S-CasHC |
| Pubmed | 24569644 |