CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0763 details


CrisprIDCrispr0763
OrganismSurrogate reporter
Gene/Locus (Literature)PPP1R1B
NCBI Gene NamePPP1R1B
Target SequenceCCATCTCAAGTCGAAGAGACCCA
Target+Mutant_Flanking

ACCATCTCAAGTCGAAGA - - -- - - - - - - - - - - - - - - - - - - - - GACCCAACCCCTGT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - D284bp - - - - - - - - - - - - - - - - - - - - TGT

Modification LengthD284
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) NA
Cell line HEK293T Cells
Method/AssayT7E1 assay/Sequencing
Crispr efficiency 23%
PRM/crRNA/VectorPRG2-CT/hU6
PRM-cas/vectorP3S-CasHC
Pubmed24569644