CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0791 details


CrisprIDCrispr0791
OrganismZea mays
Gene/Locus (Literature)ZmIPK
NCBI Gene Nameipk1A
Target SequenceCCAGGGATCCGTCTCCTTCTCCC
Target+Mutant_Flanking

CCCGACGTGTCCCCCGAGGATGACGCATCGGCCCAGGGATCCGTCTCCTTCTCCCAGGTC
CCCGACGTGTCCCCCGAGGATGACGCATCGGCCCAGGGA - - - - CCTTTCGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGAAATTACCCTTTGTTGAAAACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGTCTCCTTCTCCCAGGTC

Modification LengthD4,+125
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) 10/10ng
Cell line Protoplast
Method/AssayPCR/Sequencing
Crispr efficiency 8.90%
PRM/crRNA/VectorZmU3
PRM-cas/vector35S
Pubmed24576457