A Central Hub of Crispr based Genome Editing
CrisprID | Crispr0811 |
Organism | Mus musculus |
Gene/Locus (Literature) | Ar |
NCBI Gene Name | Ar |
Target Sequence | CCACCCTGAGAGCAGCTGCCTCCCCGAGCCTGGGGCGGCCACCGCTCCTGGCAAGG |
Target+Mutant_Flanking | AGCCTCCGAGGGCCACCCTGAGAGCAGCTGCCTCCCCGAGCCTGGGGCGGCCACCGCTCCTGGCAAGGGGCTGCCGCAGC |
Modification Length | D89 |
Genetic Modification | Deletion |
Location | Exon1 |
Conc. (ng/μl) | 10/20ng |
Cell line | Embryo |
Method/Assay | T7E1 assay |
Crispr efficiency | NA |
PRM/crRNA/Vector | pGL3-U6-sgRNA-PGK-Puro |
PRM-cas/vector | pST1374-Cas9-N-NLS-flag-linker-D10A expression vector |
Pubmed | 24584192 |