CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0854 details


CrisprIDCrispr0854
OrganismMus musculus
Gene/Locus (Literature)Rag1
NCBI Gene NameRag1
Target SequenceCCTGAATGGTCCCTTCACAGTGGTGGTAAAGGAGTCTTGCGATGGAATGGGGGATGTGAGTGAGAAGCACGGG
Target+Mutant_Flanking

ACCTGAATGGTCCCTTCACAGTGGTGGTAAAGGAGTCTTGCGATGGAATGGGGGATGTGAGTGAGAAGCACGGGA
ACCTGAATGGTCCCTTCACAGTGGTGGTAAAGGAGTCTTGCGATGGAATGGGGGATGTgaGAGTGAGAAGCACGGGA

Modification Length2
Genetic ModificationInsertion
LocationNA
Conc. (ng/μl) 10/20ng
Cell line Embryo
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpGL3-U6-sgRNA-PGK-Puro
PRM-cas/vectorpST1374-Cas9-N-NLS-flag-linker-D10A expression vector
Pubmed24584192