A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr0857 |
| Organism | Mus musculus |
| Gene/Locus (Literature) | Rag1 |
| NCBI Gene Name | Rag1 |
| Target Sequence | CCTGAATGGTCCCTTCACAGTGGTGGTAAAGGAGTCTTGCGATGGAATGGGGGATGTGAGTGAGAAGCACGGG |
| Target+Mutant_Flanking | ACCTGAATGGTCCCTTCACAGTGGTGGTAAAGGAGTCTTGCGATGGAATGGGGGATGTGAGTGAGAAGCACGGGA |
| Modification Length | 56 |
| Genetic Modification | Insertion |
| Location | NA |
| Conc. (ng/μl) | 10/20ng |
| Cell line | Embryo |
| Method/Assay | T7E1 assay |
| Crispr efficiency | NA |
| PRM/crRNA/Vector | pGL3-U6-sgRNA-PGK-Puro |
| PRM-cas/vector | pST1374-Cas9-N-NLS-flag-linker-D10A expression vector |
| Pubmed | 24584192 |