CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0859 details


CrisprIDCrispr0859
OrganismMus musculus
Gene/Locus (Literature)Rag2
NCBI Gene NameRag2
Target SequenceCCTGAACCCAGATACGGCCATTCCATTGACGTGGTGTATAGTCGAGGGAAAAGCATGG
Target+Mutant_Flanking

GAGATGTCCCTGAACCCAGATACGGCCATTCCATTGACGTGGTGTATAGTCGAGGGAAAAGCATGGGTGTTCTC
GAGATGTCCCTGAACCCAGATACGGCCATTCCATTGACGTG- - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - -

Modification LengthD215,+740
Genetic ModificationInsertion
LocationNA
Conc. (ng/μl) 10/20ng
Cell line Embryo
Method/AssayT7E1 assay
Crispr efficiency NA
PRM/crRNA/VectorpGL3-U6-sgRNA-PGK-Puro
PRM-cas/vectorpST1374-Cas9-N-NLS-flag-linker-D10A expression vector
Pubmed24584192