CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr0917 details


CrisprIDCrispr0917
OrganismHomo sapiens
Gene/Locus (Literature)CCR5
NCBI Gene NameCCR5
Target SequenceTGACATCAATTATTATACATCGG
Target+Mutant_Flanking

CAATCTATGACATCAATTATTATACATCGGAGCCCTGCCAAA
CAACTATGACATCAATTAT- - - -- - - ACACT - - - - - - - - - - - -

Modification LengthD257,+5
Genetic ModificationIndels
LocationNA
Conc. (ng/μl) NA
Cell line K562 cells
Method/AssayT7E1 assay
Crispr efficiency 65%
PRM/crRNA/VectorU6
PRM-cas/vectorCMV
Pubmed24253446