A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr1194 |
| Organism | Danio rerio |
| Gene/Locus (Literature) | gsk3b |
| NCBI Gene Name | gsk3b |
| Target Sequence | GGGACCTGACCGGCCGCAGGAGG |
| Target+Mutant_Flanking | ACTCCTGGACAGGGACCTGACCGGCCGCAGGAGGTCAGCTACACTGACA |
| Modification Length | D12,+6 |
| Genetic Modification | Indels |
| Location | NA |
| Conc. (ng/μl) | 12.5/300 |
| Cell line | Embryo |
| Method/Assay | T7E1 aasay/PCR/Sequencing |
| Crispr efficiency | 43.80% |
| PRM/crRNA/Vector | pDR299/T7 |
| PRM-cas/vector | pMLM3613 |
| Pubmed | 23874735 |