CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1196 details


CrisprIDCrispr1196
OrganismDanio rerio
Gene/Locus (Literature)gsk3b
NCBI Gene Namegsk3b
Target SequenceGGGACCTGACCGGCCGCAGGAGG
Target+Mutant_Flanking

ACTCCTGGACAGGGACCTGACCGGCCGCAGGAGGTCAGCTACACTGACA
ACTCCTGGACAGGGACCTGACCGGCCGC - - - - - - - - - - - - TACACTGACA

Modification LengthD11
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 12.5/300
Cell line Embryo
Method/AssayT7E1 aasay/PCR/Sequencing
Crispr efficiency 100%
PRM/crRNA/VectorpDR299/T7
PRM-cas/vectorpMLM3613
Pubmed23874735