A Central Hub of Crispr based Genome Editing
CrisprID | Crispr1209 |
Organism | Danio rerio |
Gene/Locus (Literature) | gsk3b |
NCBI Gene Name | gsk3b |
Target Sequence | CCTCCTGCGGCCGGTCAGGTCCCTGTCCAGGAG |
Target+Mutant_Flanking | ACCTTGGTGTCAGTGTAGCTGACCTCCTG - - - -CGGCCGGTCAGGTCCCTGTCCAGGAG |
Modification Length | NA |
Genetic Modification | NA |
Location | NA |
Conc. (ng/μl) | 12.5/200 |
Cell line | Embryo |
Method/Assay | T7E1 aasay/PCR/Sequencing |
Crispr efficiency | 6.30% |
PRM/crRNA/Vector | pDR338/T7 |
PRM-cas/vector | pMLM3613 |
Pubmed | 23874735 |