CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1209 details


CrisprIDCrispr1209
OrganismDanio rerio
Gene/Locus (Literature)gsk3b
NCBI Gene Namegsk3b
Target SequenceCCTCCTGCGGCCGGTCAGGTCCCTGTCCAGGAG
Target+Mutant_Flanking

ACCTTGGTGTCAGTGTAGCTGACCTCCTG - - - -CGGCCGGTCAGGTCCCTGTCCAGGAG
ACCTTGGTGTCAGTGTAGCTGACCTCCTGAATTCGGCCGGTCAGGTCCCTGTCCAGGAG

Modification LengthNA
Genetic ModificationNA
LocationNA
Conc. (ng/μl) 12.5/200
Cell line Embryo
Method/AssayT7E1 aasay/PCR/Sequencing
Crispr efficiency 6.30%
PRM/crRNA/VectorpDR338/T7
PRM-cas/vectorpMLM3613
Pubmed23874735