CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1213 details


CrisprIDCrispr1213
OrganismHomo sapiens
Gene/Locus (Literature)EMX1
NCBI Gene NameEMX1
Target SequenceCACGAAGCAGGCCAATGGGGAGG
Target+Mutant_Flanking

AGAAGAAGGGCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAATGGGGAGGACATCGATGTCACC
AGAAGAAGGGCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAATG - - - - - - - - ATCGATGTCACC

Modification LengthD8
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 100/500
Cell line HEK293T Cells
Method/AssaySurveyor assay/Sequencing
Crispr efficiency 38%
PRM/crRNA/VectorT7
PRM-cas/vectorT7-spCas9-NLS(D10 Cas)
Pubmed23992846