CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1218 details


CrisprIDCrispr1218
OrganismHomo sapiens
Gene/Locus (Literature)Mecp2
NCBI Gene NameMecp2
Target SequenceAAGCTTAAACAAAGGAAGTCTGG
Target+Mutant_Flanking

GATGACCCCACCTTGCCTGAAGGTTGGACACGAAAGCTTAAACAAAGGAAGTCTGGCCGATCTGCTGGAA
GATGACCCC - - -TTGCCTGAAGGTTGGACACGAAAGCTTAAACAAAGGA - - - - - - - - - - - TCTGCTGGAA

Modification LengthD3,D11
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 50/100
Cell line HEK293T Cells
Method/AssaySurveyor assay/Sequencing
Crispr efficiency 38%
PRM/crRNA/VectorT7
PRM-cas/vectorT7-spCas9-NLS(D10 Cas)
Pubmed23992846