A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr1224 |
| Organism | Homo sapiens |
| Gene/Locus (Literature) | Mecp2 |
| NCBI Gene Name | Mecp2 |
| Target Sequence | AAGCTTAAACAAAGGAAGTCTGG |
| Target+Mutant_Flanking | GATGACCCCACCTTGCCTGAAGGTTGGACACGAAAGCTTAAACAAAGGAAGTCTGGCCGATCTGCTGGAA |
| Modification Length | D3 |
| Genetic Modification | Deletion |
| Location | NA |
| Conc. (ng/μl) | 50/100 |
| Cell line | HEK293T Cells |
| Method/Assay | Surveyor assay/Sequencing |
| Crispr efficiency | 38% |
| PRM/crRNA/Vector | T7 |
| PRM-cas/vector | T7-spCas9-NLS(D10 Cas) |
| Pubmed | 23992846 |