A Central Hub of Crispr based Genome Editing
CrisprID | Crispr1225 |
Organism | Homo sapiens |
Gene/Locus (Literature) | Mecp2 |
NCBI Gene Name | Mecp2 |
Target Sequence | AAGCTTAAACAAAGGAAGTCTGG |
Target+Mutant_Flanking | GATGACCCCACCTTGCCTGAAGGTTGGACACGAAAGCTTAAACAAAGGAAGTCTGGCCGATCTGCTGGAA |
Modification Length | D3 |
Genetic Modification | Deletion |
Location | NA |
Conc. (ng/μl) | 50/100 |
Cell line | HEK293T Cells |
Method/Assay | Surveyor assay/Sequencing |
Crispr efficiency | 38% |
PRM/crRNA/Vector | T7 |
PRM-cas/vector | T7-spCas9-NLS(D10 Cas) |
Pubmed | 23992846 |