CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1226 details


CrisprIDCrispr1226
OrganismHomo sapiens
Gene/Locus (Literature)Mecp2
NCBI Gene NameMecp2
Target SequenceAAGCTTAAACAAAGGAAGTCTGG
Target+Mutant_Flanking

GATGACCCCACCTTGCCTGAAGGTTGGACACGAAAGCTTAAACAAAGGAAGTCTGGCCGATCTGCTGGAA
GATGACCCCACCTTGCCTGAAGGTTGGACACGAAAGCTTAAACAAAGG
- - - TCTGGCCGATCTGCTGGAA

Modification LengthD3
Genetic ModificationDeletion
LocationNA
Conc. (ng/μl) 50/100
Cell line HEK293T Cells
Method/AssaySurveyor assay/Sequencing
Crispr efficiency 38%
PRM/crRNA/VectorT7
PRM-cas/vectorT7-spCas9-NLS(D10 Cas)
Pubmed23992846