A Central Hub of Crispr based Genome Editing
| CrisprID | Crispr1391 |
| Organism | Toxoplasma gondii |
| Gene/Locus (Literature) | SAG1 |
| NCBI Gene Name | SAG1 |
| Target Sequence | GGCAGTGAGACGCGCCGTCACGG |
| Target+Mutant_Flanking | CCGAAGGCAGTGAGACGCGCCG - - - - -- - - - - - - - - - - - - - - TCACGGCAGGGG |
| Modification Length | 31 |
| Genetic Modification | Insertion |
| Location | NA |
| Conc. (ng/μl) | NA |
| Cell line | HFF |
| Method/Assay | TOPO Cloning/PCR/Sequencing |
| Crispr efficiency | NA |
| PRM/crRNA/Vector | SNR52 |
| PRM-cas/vector | pX330 |
| Pubmed | 24971596 |