CrisprGE


     A Central Hub of Crispr based Genome Editing



Crispr1409 details


CrisprIDCrispr1409
OrganismBombyx mori
Gene/Locus (Literature)Bm-ok
NCBI Gene NameBm-ok
Target SequenceGGAGATCGGCGGTCTATGAAGAGG
Target+Mutant_Flanking

GGGAGTGATGGGAGATAGCCCGG . . . 66bp . . . GGAGATCGGCGGTCTATGAAGAGG
 - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - ggttGAGG

Modification LengthD130,+4
Genetic ModificationIndels
LocationExon1
Conc. (ng/μl) 200/500
Cell line Fertilized eggs
Method/AssayTA cloning/Sequencing
Crispr efficiency 31.60%
PRM/crRNA/VectorT7
PRM-cas/vectorSp6
Pubmed25013902